WebWe generated advillinCre/+ ;CB2f/f conditional knockout mice to ascertain the role of CB2 localized to primary sensory neurons in CB2-mediated therapeutic effects. Antinociceptive efficacy of both AM1710 and LY2828360, but not reference analgesics, were absent in advillinCre/+ ;CB2f/f mice, which exhibited robust ddC-induced neuropathy. WebOn average, less than one positive event per entire reaction volume was detected in both control and mutant samples. A control sample of DRG tissue from an AdvillinCre-Trpa1fl/fl mouse shows that Cre-mediated recombination in a different tissue (sensory neurons) reduces Trpa1 mRNA detection (DRGAdv).

Deletion of PIK3C3/Vps34 in sensory neurons causes - JSTOR

Webin all sensory neurons. (A Left) Photo of a control heterozygous (Advillincre,+; Pik3c3flox/+) and two mutant (Advillincre/+; Pik3c3flox/flox) mice. (Right) PCR and Southern blotting confirmed deletion of exon 17-18. (B) Fluorescent in situ hybridization (green) and immunostaining of PIK3C3 (red) in control and Pik3c3 WebMay 30, 2014 · Initially described as chromatolysis or cell body reaction, injury to peripheral nerves triggers a series of morphologic and biochemical changes in neuronal cell bodies ( 2 , 3 ), including increases in RNA content and in the rate of protein synthesis ( 4 , 5 ). how to minimise percentage uncertainty

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WebStrain #: 014143 RRID:IMSR_JAX:014143 Common Name: Lkb1fl Lkb1 fl mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 ( Stk11) gene. Exposure to Cre … Webpreviously described Piezo2 knockout mouse lines, AdvillinCre; Piezo2cKO (Piezo2iAdv) and HoxB8Cre;Piezo2KO (Piezo2HoxB8), to determine whether constitutive Cre … how to minimise power in therapy