WebApr 11, 2024 · To detect EGFP, blots were incubated with primary rabbit anti-GFP antibody (1:3000 dilution, A-11122, Fisher-Scientific, San Francisco, CA) and mouse anti-beta Tubulin [E7] (1:500 dilution, hybridoma bank, IA) diluted in blocking solution overnight at 4 degrees, followed by secondary goat anti-rabbit 680 and anti-mouse 800 nm at 1:15000 ... WebGFP Antibody [IQGFP6 Polyclonal] is a Chicken/Bird Polyclonal antibody and is validated in E and WB. Your Cart. ... Dilutions: Optimal antibody dilution should be determined by titration. Also Known As: ... Western …
GFP Antibody Cell Signaling Technology
WebTested Applications Dilution * Western Blot (WB) 1:500 - 1:2000 Immunocytochemistry (ICC) 1:200 - 1:1000 ELISA (ELISA) 1:500 - 1:2000 * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. For Research Use Only. WebTransfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 50430-2-AP (GFP tag Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. IP Result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:5000) with Transfected HEK-293 cells lysate 300ug. jb whitney \\u0026 associates
Chicken anti-GFP, Polyclonal, Novus Biologicals™ - Fisher Sci
WebApr 10, 2024 · ApoE2 increases α-CTF and decreases Aβ production in a cell-autonomous manner. To investigate the potential cell-autonomous effects of ApoE on APP cleavage, APP was co-expressed with ApoE in a neuronal cell line (N2A; Figure 1 C) and HEK 293T cells (Figure S2 C). α-C-terminal fragment (α-CTF) is a non-amyloidogenic cleavage … WebUse the following western detection protocol with rabbit and chicken anti-GFP antibody conjugates. Be sure to use enough solution in an appropriate container to completely ... WebRestoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury. In Research (Washington, D.C.) on 18 March 2024 by Gal, L., Bellák, T., et al.. jb whitney and associates