2024 Ift88 cilia

Ift88 cilia

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August undefined, 2024

WebДиетология с особым цинизмом WebThis study examines the role of the intraflagellar transport (IFT) protein, IFT88, in regulating fundamental actin organisation and mechanics in articular chondrocytes. Methods: The …

Functional aspects of primary cilia in signaling, cell cycle and ...

Web13 jul. 2024 · A model for IFT88 mediated secretory pathway from ER to primary cilia. IFT88 is found at the ER exit sites, where it is incorporated into the ER-derived COP II-coated … Web28 jun. 2024 · Ift88 deletion in mice results in defective ciliogenesis and cystic disease., The primary cilia integrate cellular responses to regulate cell proliferation, differentiation, migration, and polarity. 15 Cilia removal by deleting Kif3a (encoding kinesin-II) suppressed cyst growth in both Pkd1 and Pkd2 knockout (KO) mouse models. highs north east md

Intraflagellar transport complex structure and cargo interactions - Cilia

WebImmunofluorescence analysis of IFT88 in HepG2 cells using a IFT88 monoclonal antibody ( Product # PA5-18467) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing nuclear and Golgi/cytoplasmic staining. Web1 mrt. 2024 · In analyses of chick heart development, cilia were observed in differentiating myoblasts, myocytes, fibroblasts, and fibrocytes. 18 Subsequent studies described cardiac primary cilia in embryonic and adult human heart 14, 19 and in aortic endothelial cells. 20 However, it remains unclear which cardiac cell types express cilia and whether this … Web19 dec. 2024 · To determine whether aldosterone affects ciliary lengthening through its effects on IFT, we investigated protein levels and degradation of the core IFT complex B protein, IFT88 and the molecular motor, KIF3A, which are involved in anterograde transport and ciliary growth, respectively. 34, 35 Aldosterone treatment increased IFT88 protein … small seed envelopes

Pilar Okenve-Ramos, PhD. - Postdoctoral Research Scientist

WebDescription: Homo sapiens nephrocystin 1 (NPHP1), transcript variant 4, mRNA. (from RefSeq NM_001128179) RefSeq Summary (NM_001128179): This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell … Web14 okt. 2015 · Ift88 is an essential protein within cilia that has played a pivotal role in unveiling the function of cilia in mammalian development and disease . The Oak Ridge … small seed envelope wholesaleWebHigh speed video microscopy of cultured patient epithelial cells showed severely dyskinetic cilia, but no obvious ultra-structural abnormalities on routine transmission electron microscopy (TEM). Further investigation revealed that LRRC56 interacts with the intraflagellar transport (IFT) protein IFT88. small seed space in prng

"Web15 feb. 2010 · J Cell Sci (2010) 123 (4): 499–503. The primary cilium, which was first so named by Sergei Sorokin ( Sorokin, 1968 ), is a solitary organelle that emanates from the cell surface of most mammalian cell types during growth arrest. Increasing evidence suggests that primary cilia are key coordinators of signaling pathways during … " - Ift88 cilia

Ift88 cilia

Primary cilia dynamics instruct tissue patterning and repair of …

WebWe found that, while over 60% of the wild-type PE cells were ciliated, in Ift88 KO mice only 3% of cells were ciliated and Ift20 KO embryos lack cilia. We compared the PE volume of Ift20 and Ift88 KO mice to their con- trols, using an anti-Wt1 antibody (a well-established PE marker; Carmona et al., 2001). Web19 jun. 2024 · Here, we report that disruption of intraflagellar transport protein 88 (IFT88), a core ciliary trafficking protein, increases chondrocyte aggrecanase activity in vitro.We find that the receptor for protease endocytosis in chondrocytes, LDL receptor-related protein 1 (LRP-1), is unevenly distributed over the cell membrane, often concentrated at the site of …

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WebPrimary cilia and associated proteins Intraflagellar transport (IFT) complex B1 K16474 IFT88; intraflagellar transport protein 88. BRITE hierarchy: Genes: HSA: 8100(IFT88) ... Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. Journal: J Cell Biol 151:709-18 (2000) Web4 apr. 2024 · Ciliary IFT88 Protects Coordinated Adolescent Growth Plate Ossification From Disruptive Physiological Mechanical Forces. Multiomic identification of factors associated …

Web2 apr. 2013 · Ciliary expression was induced by serum starvation and assessed by staining with acetylated α-tubulin, IFT88, and the centrosome marker, γ-tubulin; our results showed that only 3% (Hucct-1) and 1.8% (KMCH) of the cholangiocarcinoma cell lines express cilia, whereas in the normal cell lines, cilia were found in 67% (H69) and 61% (NHC) of the … Web18 apr. 2013 · To initiate cilia ablation in adult mice, a previously described conditional allele of Ift88 and ubiquitously expressed inducible CAGG-CreER line were used ( 22, 23 ). Cohorts were generated such that transgene CAGG-CreER–positive animals were always compared with control CAGG-CreER–negative littermates.

WebRegulation of Primary Cilia Length by O-GlcNAc during Neuronal Development in Human Neuron Model Jie L. Tian 1,2 ... 12, 25, 34, 45, and 55 and stained for IFT88, Arl13b, and NeuN (after day 25). Cilia lengths were measured on NeuN-positive cells after day 25. Scale bars, 40 μm. All data are mean ± SD from three independent experiments (> 80 ... Web1 mrt. 2024 · Cilia of olfactory sensory neurons (OSNs) are the primary site of odor binding; hence, their loss results in anosmia, ... We utilized OSN-specific Ift88 knockout mice ...

Web22 nov. 2024 · The role of primary cilia in enamel production has also been evaluated using a conditional deletion of Ift88 in epithelial cells (Keratin14; Ift88 fl/fl) . K14 ; Ift88 fl/fl mice had chalky incisors and roughened enamel surfaces on all molars due to abrasions that became more severe with time.

WebThe intraflagellar transport (IFT) machinery, including IFT88 in cilia, is involved in the maintenance of bidirectional motility along the axonemes, which is required for … small seed hand seederWeb28 feb. 2024 · Dual immunofluorescence showed that IFT88 colocalized with Arl13b along cilia . Ift88 knockdown notably reduced the percentage of cells that were ciliated and the expression of Prl8a2 . Collectively, these data demonstrate that primary cilia were required for mouse decidualization in vitro and in vivo. highs odentonWebIft88, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. ... Article Title: Characterization of Primary Cilia Formation in … highs of baltimore mdWebAbstract Loss of nephron primary cilia due to disruption of the Ift88 gene results in sex‐ and age‐specific phenotypes involving renal cystogenesis, blood ... Profiling renal sodium transporters in mice with nephron Ift88 disruption: Association with sex, cysts, and blood pressure Chunyan Hu, Jayalakshmi Lakshmipathi, small seed of grain dispenserWeb27 mrt. 2011 · One of the best-studied IFT proteins, IFT88, which was first characterized for its role in cilia formation and polycystic kidney disease 3, 16, 17, 18, 19, also localizes to … highs of baltimore logoWebIFT88/primary cilia play a role in metabolic reprogramming in thyroid cancer cells. BFGF also upregulated the mRNA and protein expression of IFT88 in primary cultured … highs optimization solverWeb31 dec. 2024 · We analyzed cochleae from three ciliopathy mouse models exhibiting different ciliogenesis defects: Intraflagellar transport 88 ( Ift88 ), Tbc1d32 (a.k.a. bromi ), and Cilk1 (a.k.a. Ick) mutants. These mutants showed multiple developmental defects including shortened cochlear duct and abnormal apical patterning of the organ of Corti. highs of baltimore llc