Imod ctfphaseflip
Witryna5 lis 2024 · Tomograms were reconstructed in IMOD using weighted back-projection, with a simultaneous iterative reconstruction technique (SIRT)–like filter to aid visualization and segmentation. Defocus-contrast tomograms were contrast transfer function corrected in IMOD using ctfphaseflip. VPP tomograms were left uncorrected. WitrynaFurther analysis of the maintenance status of scipion-em-imod based on released PyPI versions cadence, the repository activity, and other data points determined that its …
Imod ctfphaseflip
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Witrynapatch2imod: Make an IMOD model of displacement vectors from a patch displacement file. point2model: Convert point listing to IMOD model file. ... ctfphaseflip: Corrects tilt … Witryna1 gru 2009 · CTFPHASEFLIP corrects images line-by-line by inverting phases appropriately in thin strips of the image at nearly constant defocus. CTF correction by these methods is shown to improve the resolution of aligned, averaged particles extracted from tomograms. ... IMOD is useful for studying and modeling data from …
Witryna4 lut 2024 · The CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62. Tomograms were manually segmented using AMIRA (Thermo Fisher Scientific) or ... Witryna4/2/10: Fixed initialization problem in Ctfphaseflip's detection of bad view numbers and fixed the warning when a simple one-line file is used. 4/1/10: Fixed bug in Ctfplotter …
Witryna1 gru 2009 · CTFPHASEFLIP corrects images line-by-line by inverting phases appropriately in thin strips of the image at nearly constant defocus. CTF correction by these methods is shown to improve the resolution of aligned, averaged particles extracted from tomograms. ... This paper has described methods implemented in … WitrynaExposure-filtered images were then used for tomogram reconstruction in the IMOD software package (39). CTF-correction was performed by the “ctfphaseflip”-program implemented in IMOD (40). CTF-corrected unbinned tomograms were reconstructed and VLPs and virus particles were identified in the 8x-binned tomograms using the Amira
Witryna1 wrz 2024 · The 2D strip-based approach performed using IMOD’s ctfphaseflip is slower than the simple angle-independent CTF correction used in NovaCTF for a …
Witrynaimod.protocols.protocol_ctfCorrecion module¶ class imod.protocols.protocol_ctfCorrecion.ProtImodCtfCorrection (**kwargs) [source] ¶. Bases: pwem.protocols.protocol ... the official scratchjr bookWitryna1 lip 2012 · Local approaches, however, are currently the standard in the field. For example the program CTFPHASEFLIP [6] as incorporated in the widely used software package IMOD [16] ... Projection after (local) tilted phase flipping using CTFPHASEFLIP, which is part of IMOD. (D) Difference between (B) and (C). The tilt-axis is horizontal. … mickey and minnie mouse relatedWitryna25 wrz 2024 · Defocus estimation was performed using IMOD ctfplotter , phase flipping was performed using NovaCTF or ctfphaseflip, and dose weighting was performed using IMOD mtffilter. Tomograms were aligned using IMOD etomo and subsequently reconstructed using NovaCTF, with the exception of eight tomograms that were used … mickey and minnie mouse safariWitrynaIMOD_TMPDIR Path for temporary directory used by scripts. On Windows, this can be in Cygwin or Windows format, with forward or back slashes. IMOD_USE_GPU IMOD_USE_GPU2 These variables will tell the Tilt and Ctfphaseflip programs to use the GPU on a CUDA-capable NVIDIA graphics card. Set the variable to 0 to use the best … mickey and minnie mouse slippersWitrynaBug Fixes for IMOD 4.11.6 4/30/21: Fixed Ctfphaseflip crash when nx = 4092 at 0 degrees. 4/15/21: Fixed final output file name from Ctf3dsetup with new naming style. … mickey and minnie mouse stencilsWitrynaCTF correction: CTF correction of a set of input tilt-series using the IMOD procedure. More info: ctfphaseflip doc; Dose filter: Tilt-series dose filtering based on the IMOD … the official site of jenn air appliancesWitryna1 lip 2016 · Tilt series alignment and tomogram reconstruction were done using the batch reconstruction protocol of etomo in IMOD software (version 4.12.10; [56, 57] ). … mickey and minnie mouse purses