Laminin overlay assay
WebbThe laminin-binding activity of a-DG was also nearly abolished in the mutant skeletal and cardiac muscles as demonstrated by laminin overlay assay ( Fig. 4A and D). Webb23 apr. 1999 · Biotin-labeled laminin-1 overlay assay of dissociated BAE membrane proteins indicated the interaction of laminin-1 with alphaDG. The laminin alpha5 …
Laminin overlay assay
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WebbSLC35A1 KO cells still retained some signal in the laminin overlay assay (Fig. 1H) and in flow cytometry with the IIH6 antibody, which recognizes the intact α-dystroglycan glycan (Fig. 1, D and G). This indicates that while SLC35A1 might be the major transporter for CDP-ribitol in HAP1 cells, other transporters might contribute. Webb17 aug. 1999 · Overlay Assay for rML-LBP21 Binding to Endogenous Bovine Peripheral Nerve Laminin-2. The nitrocellulose transfers of rML-LBP21 or M. leprae cell wall were blocked with 10 mM triethanolamine (pH 7.6), 140 mM NaCl, 1 mM CaCl 2 , and 1 mM MgCl 2 (LBB) containing 5% nonfat dry milk (MLBB) and then incubated with 10 ml …
Webb8 feb. 2008 · To search for an associated protein that conceals the nonsulfated HNK-1 epitope on laminin-1, we carried out an overlay assay analysis. Thus, a mouse kidney homogenate was biochemically fractionated and then subjected to laminin-1 overlay assaying (Figure 5A). For this method, anti-laminin-1 pAb was used to detect the … Webb1 dec. 2015 · WGA-purified preparations from differentiated (d-Kasumi-1) and non-differentiated (nd-Kasumi-1 cells) compartments of Kasumi-1 cells were analysed by …
Webb25 maj 2024 · Ligand overlay assays were performed on PVDF-FL membranes using mouse Engelbreth-Holm-Swarm (EHS) laminin (Thermo Fisher Scientific, 23017015). Briefly, PVDF-FL membranes were blocked in laminin binding buffer [LBB; 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 (pH 7.6)] containing 5% …
Webb1 maj 2004 · In lane 1, as a positive control, we show a laminin overlay assay followed by a western blot with anti-laminin antibody. As expected, if the laminin overlay …
Webb24 juni 2024 · The matriglycan-engineered fetuin’s with 4 and 5 repeating units (10d and 10e) were also capable of binding recombinant mouse laminin α1 (LG4-LG5 domains) in an overlay assay (Fig. 3c). helper\\u0027s highWebb20 mars 2012 · Given the high level of Itgb1 and laminin in the ventricular and subventricular regions 19, we have examined whether laminin is important for directional axon growth in this assay. helper tower for toddlersWebb17 apr. 2024 · Laminin is an essential extracellular matrix component that binds with glycosylated α-DG to guide astrocyte migration and maintain the ILM integrity . We … helper\u0027s choice hong kongWebb14 jan. 2024 · Integrin ligands in the TSC niche in vivo. We examined the ECM niche profile around TSCs by in situ integrin overlay assays, in which soluble recombinant integrins were able to bind and visualize their ligands in situ (Kiyozumi et al, 2012, 2014, 2024).We applied the assays to E5.5 embryonic sections because TSCs were shown … helper track upsWebb25 juni 2024 · WGA-AE purification, immunoblot analysis of glycoproteins and laminin overlay assay Whole cell protein lysates were prepared from sub-confluent cultures using WGA Lysis Buffer (50 mM Tris/HCl, pH 7.4 (Sigma), 150 mM NaCl (Carl Roth), 1% Triton X-100 (AppliChem, Darmstadt, Germany) and Protease Inhibitors (Sigma, Merck), and … helper tower for kidsWebbThe extensive glycosylation induced by LARGE was detected by laminin overlay assay and immunoblotting with IIH6 mAb, as a broad and high molecular band (Fig. 3A). However, when α-DG-Fc was co-transfected with HNK-1ST-EGFP, there was a remarkable decrease in the laminin binding activity and almost complete loss of IIH6 … helper trucks puppyWebb1 feb. 2012 · The laminin overlay assay did not show laminin-binding activity. However, overexpressing LARGE in POMT2 KO neural stem cells resulted in IIH6C4 … laminate floor in basement